Plasma Proteins: Some Chemical Methods of Fractionation; Starch Gel Electrophoretic Analysis

Electrophoretic Analysis of Plasma and Urinary Proteins.

Accurate analysis of the protein fractions of human plasma has in the past been very difficult and time-consuming. Fractional precipitation by neutral salt, as in Howe's method (1), has proved useful because of its simplicity and because the most important pathologic variations in plasma proteins involve a gross diminution in the albumin fraction. Such methods have definite limitations: Butler'...

Behavior of the Serum proteins in starch-agar-gel electrophoresis

Fractionation of Bence-Jones protein by starch gel electrophoresis.

The development by Smithies (1955a) of starch gel as a medium for the electrophoresis of protein material marked an important advance, as it combines separation by electrical mobility with a form of pore filtration. Smithies (1955b) has been able to obtain 12 or more protein fractions from human serum with his one-dimensional technique and equally remarkable separation has been obtained with an...

Starch-gel electrophoresis of rat-serum proteins.

In 1955, Smithies (1) described a procedure for the separation of human serum proteins by horizontal zone electrophoresis in starch gels. The separation of zones was improved by a later modification to a vertical electrophoresis in which the sample was inserted in the gel, free from supporting medium (2). With these methods, it has been demonstrated that many more protein fractions can be separ...

Electrophoretic Analysis of the Proteins of Plasma and Serous Effusions.